Structural analysis of a eukaryotic sliding DNA clamp
Jun 17, 2004 · Indeed, comparison of the inactive form of the E. coli clamp loader complex 11,30 with the RFC structure shows that the former can be described as a distorted and open form of the RFC spiral
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Researchers identify how the bacterial replic | EurekAlert!
Feb 26, 2019 · Here, the helicase -- helicase loader complex is shown atop a set of cryogenic electron microscopy images used to visualize the structure. view more Credit: Jillian Chase and David Jeruzalmi
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Structure of a helicase–helicase loader complex reveals
Sep 19, 2013 · The helicase–helicase loader complex exhibits at least two different quaternary structures in solution.
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EM structure of a helicase-loader complex depicting a 6: 2
Lin, Y-C, Naveen, V & Hsiao, C-D 2016, ' EM structure of a helicase-loader complex depicting a 6: 2 binding sub-stoichiometry from Geobacillus kaustophilus HTA426 ', Biochemical and Biophysical Research Communications, vol. 473, no. 1, pp. 243-248.
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Crystal Structure of the Processivity Clamp Loader Gamma
The γ complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (β, homologous to PCNA) onto DNA. The 2.7/3.0 Å crystal structure of γ complex reveals a pentameric arrangement of subunits, with stoichiometry δ′:γ3:δ. The C-terminal domains of the subunits form a circular collar that supports an asymmetric arrangement of the
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Crystal structure of the complex of the interaction
(A) Overall structure of the helicase loader–EcDnaB C –EcDnaB L ' complex. (B) The intermolecular three α-helix bundle formed in the helicase loader–EcDnaB C –EcDnaB L ' complex. The two helices, B L '-α1 and B C-α5, are coloured green and purple, respectively.
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Insights into the structure and assembly of the Bacillus
The correct stoichiometry of the clamp-loader complex from a Gram-positive organism is revealed for the first time and new insights into its structure are presented, including a homology model of the τ 3-δ-δ′ core complex indicating the relative spatial position of the unknown Cτ domain. Additionally, the X-ray crystal structure of the δ
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Researchers identify how the bacterial replicative
Feb 26, 2019 · Here, the helicase -- helicase loader complex is shown atop a set of cryogenic electron microscopy images used to visualize the structure. Credit: Jillian Chase and David Jeruzalmi
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Loading strategies of ring-shaped nucleic acid
Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly. Nat Commun. 2013; 4:2495. This study presents a crystal structure of a ternary primosomal complex of the G. stearothermophilus DnaB helicase and the helicase binding domain of DnaG primase bound to B. subtilis DnaI loader protein
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The Bacterial DnaC Helicase Loader Is a DnaB Ring Breaker
structure of the intact E. coli DnaB,DnaC helicase/ loader complex. The 480 kDa dodecamer forms a three-tieredassembly,inwhichDnaCadoptsaspiral configuration that remodels N-terminal scaffolding and C-terminal motor regions of DnaB to produce a clear break in the helicase ring. Surprisingly, DnaC's AAA+ fold is dispensable for ring remodeling
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PDBe < PISA < EMBL-EBI
mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex Interfaces XML View Details Download Search Structure 1
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Structural analysis of a eukaryotic sliding DNA clamp
Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA).
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EM structure of a helicase-loader complex depicting a 6:2
PDB-101: Molecule of the Month: DNA Helicase
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EM structure of a helicase-loader complex depicting a 6:2
Apr 22, 2016 · The EM structure of the captured 6:2 GkDnaC-GkDnaI complex exhibits features (such as the un-opened helicase ring and the DnaI density capable of blocking the central channel) that could potentially act as a checkpoint conformation to prevent the loading of immature helicase-loader complex onto DNA (see Fig. 4). These findings extend our
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Researchers Identify How the Bacterial Replicative
Feb 26, 2019 · Here, the helicase — helicase loader complex is shown atop a set of cryogenic electron microscopy images used to visualize the structure. Image credit: Jillian Chase and David Jeruzalmi "Going into this research, we knew there had to be a loader protein for this action to take place, but we didn't know what the process looked like
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Structure of a helicase-helicase loader complex reveals
Dec 01, 2012 · The helicase–helicase loader complex exhibits at least two different quaternary structures in solution. These two different conformations include a spiral helicase in complex with spiral helicase loader5, and the open-ring helicase bound with the open-ring helicase loader4. The former is probably the preferred conformation that allows helicase loading onto ssDNA.
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Crystal structure of the complex of the interaction
Sep 01, 2013 · Here, to provide a better understanding of this key process, we report the crystal structure of the ~570-kDa prepriming complex between the Bacillus subtilis loader protein and the Bacillus stearothermophilus helicase, as well as the helicase-binding domain of primase with a molar ratio of 6:6:3 at 7.5 Å resolution.
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Structure of a helicase–helicase loader complex …
Structure of a helicase–helicase loader complex reveals
Get a quoteStructure and primase-mediated activation of a bacterial
Transition from a loaded helicase•helicase-loaders•primase complex to an active unwinding primosome requires additional conformational changes of the helicase. Closing of the helicase rings is likely facilitated by nucleotide and ssDNA binding since the translocation conformation of DnaB …
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Crystal structure of DnaT84–153-dT10 ssDNA complex reveals
Aug 18, 2014 · INTRODUCTION. A faithful and reliable transmission of genetic material requires precise coordination and regulation of chromosome replication (1,2).This process requires the assembly of a replication complex, a primosome, at the origin ().A primosome consists of several proteins, including helicase, primase and several auxiliary proteins that are responsible for creating RNA primers on …
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